Measles IgG capture EIA: Cat. No: MeVG011

Intended use and application:

Enzyme immunoassay (EIA) for the detection of human IgG antibodies to measles virus in serum / plasma and oral fluid is intended to be used as an aid to diagnosis of measles infection and for the epidemiological surveillance of measles antibodies in population studies.

Test principle:

In the Microimmune measles IgG capture EIA, undiluted oral fluid extracted from a collection device (e.g "Oracol") or diluted serum / plasma is added to anti-human IgG coated microtitre wells. IgG in the specimen binds to the wells and after washing, recombinant measles nucleoprotein (rMVN) antigen is added. Measles specific IgG in the sample, if present, binds the rMVN. After washing the wells, a monoclonal antibody to the rMVN conjugated to horseradish peroxidase is added. After washing, tetramethylbenzadine (TMB) substrate is added to reveal the presence of specific IgG. The presence of measles specific IgG results in a colour change in the TMB from colourless to blue and then yellow on terminating the enzymatic reaction. The colour change and intensity is monitored using a spectrophotemetric plate reader set at 450nm with a reference filter between 620 and 650nm. The presence of measles specific IgG is indicated by optical density values above the cut-off.

Features of Test:

• Assay time: < 2 h
• Specimen type: oral fluid, serum / plasma
• Reagents: Colour coded and mostly ready to use.
• Shelf life: 12 months at 2..8°C
• *Sensitivity of the Microimmune Measles IgG test on oral fluid compared to the serum results using the same test was 92.9% (95% CI 84.1 and 97.6%) and respectively.
• *Specificity of the Microimmune Measles IgG test on oral fluid compared to the serum results using the same test was 97.7% (95% CI 87.9 and 99.9%)
• *PPV on oral fluid samples compared to serum test results: 98.5 (95% CI 91.8 and 100.0%)
• *NPV on oral fluid samples compared to serum test results: 89.6% (95% CI 77.3 and 96.5%) *A detailed description of the evaluation of the test is given in the product insert.

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