|Measles IgG Capture Assay|
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Measles IgG capture EIA: Cat. No: MeVG011
Intended use and application:
Enzyme immunoassay (EIA) for the detection of human IgG antibodies to measles virus in serum / plasma and oral fluid is intended to be used as an aid to diagnosis of measles infection and for the epidemiological surveillance of measles antibodies in population studies.
In the Microimmune measles IgG capture EIA, undiluted oral fluid extracted from a collection device (e.g "Oracol") or diluted serum / plasma is added to anti-human IgG coated microtitre wells. IgG in the specimen binds to the wells and after washing, recombinant measles nucleoprotein (rMVN) antigen is added. Measles specific IgG in the sample, if present, binds the rMVN. After washing the wells, a monoclonal antibody to the rMVN conjugated to horseradish peroxidase is added. After washing, tetramethylbenzadine (TMB) substrate is added to reveal the presence of specific IgG. The presence of measles specific IgG results in a colour change in the TMB from colourless to blue and then yellow on terminating the enzymatic reaction. The colour change and intensity is monitored using a spectrophotemetric plate reader set at 450nm with a reference filter between 620 and 650nm. The presence of measles specific IgG is indicated by optical density values above the cut-off.
Features of Test:
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