Measles IgM Capture EIA:Cat No: MeVM010

Intended use and application:

Enzyme Immunoassay (EIA) for the detection of human IgM antibodies to measles virus in serum and plasma, and oral fluid.

Test principle:

In the Microimmune Measles IgM capture EIA, undiluted, extracted oral fluid (e.g "Oracol" or "Orasureâ") or diluted serum / plasma is added to anti-human IgM coated microtitre wells. IgM in the specimen binds to the wells and after washing, recombinant measles nucleoprotein (rMeNP) antigen is added. Measles specific IgM in the sample, if present, binds the rMeNP. After washing the wells, a monoclonal antibody to the rMeNP conjugated to horseradish peroxidase is added. After washing, tetramethylbenzadine (TMB) substrate is added to reveal the presence of specific IgM. The presence of measles specific IgM results in a colour change in the TMB from colourless to blue and then yellow on terminating the enzymatic reaction. The colour change and intensity is monitored using a spectrophotemetric plate reader set at 450nm with a correction filter between 620 and 650nm. The presence of measles specific IgM is indicated by optical density values above the cut-off.

Features of Test:

• Assay time: < 2 h
• Specimen type: oral fluid, serum / plasma
• Reagents: Colour coded and mostly ready to use.
• Shelf life: 12 months at 2..8°C
• Sensitivity:100% on serum specimens compared to an IgM capture radioimmunoassay (MACRIA);
• Sensitivity 100% on oral fluid specimens compared to MACRIA using serum/oral fluid pairs (see product insert).
• Specificity: 96.1% on serum specimens compared to MACRIA.

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